ManuScript Details
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Paper Id:
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IJARW3147
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Title:
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MOLECULAR DETECTION AND SPECIES IDENTIFICATION OF EIMERIA SPP. IN BROILER FARMS NEAR BY ANAND, GUJARAT, INDIA
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| Published in: |
International Journal Of All Research Writings |
| Publisher: |
IJARW |
| ISSN: |
2582-1008 |
| Volume / Issue: |
Volume 8 Issue 1 |
| Pages: |
5
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| Published On: |
7/8/2026 11:39:51 PM (MM/dd/yyyy) |
Main Author Details
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Name:
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Thakare Jatinbhai Ramchandrabhai |
| Institute: |
College of Veterinary Science and Animal Husbandry Anand KU |
Co - Author Details
| Author Name |
Author Institute |
| Maulik rajvi |
College of Veterinary Science and Animal Husbandry Anand Ku |
| Dhwani R. Oza |
College of Veterinary Science and Animal Husbandry Anand Ku |
| Nilima N. Brahmbhat |
College of Veterinary Science and Animal Husbandry Anand Ku |
| Vandip D. Chauhan |
College of Veterinary Science and Animal Husbandry Anand Ku |
Abstract
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Research Area:
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Veterinary Sciences |
| KeyWord: |
Broiler, Coccidiosis, Eimeria, PCR, ITS-1, Molecular diagnosis, Gujarat |
| Abstract: |
Coccidiosis is one of the most economically important protozoan diseases affecting the
poultry industry worldwide. The present study was undertaken to determine the
prevalence and molecular characterization of Eimeria species infecting commercial
broiler farms in Anand district, Gujarat, India. A total of 22 broiler farms comprising 400
birds were surveyed. Faecal and litter samples were collected and examined using
flotation and sedimentation techniques. Positive samples were subjected to sporulation
using 2.5% potassium dichromate solution and subsequently analyzed by polymerase
chain reaction (PCR) for species identification. Microscopic examination revealed that 15
out of 22 farms were positive for coccidiosis, indicating an overall prevalence of 68.19%.
Molecular confirmation using genus-specific primers targeting the cytochrome oxidase I
(COI) gene amplified an approximately 810 bp fragment confirming the presence of
Eimeria species. Species-specific PCR targeting the internal transcribed spacer-1 (ITS-1)
region identified six Eimeria species, namely Eimeria acervulina (321 bp), Eimeria
brunetti (311 bp), Eimeria mitis (306 bp), Eimeria necatrix (285 bp), Eimeria tenella (278
bp), and Eimeria maxima (145 bp). Eimeria tenella was the predominant species,
occurring as a single infection in 40% of positive farms, while mixed infections were
observed in the remaining farms. The findings indicate that coccidiosis remains highly
prevalent in broiler farms of Central Gujarat and that PCR-based diagnosis provides a
sensitive and reliable approach for species identification. Routine molecular surveillance
coupled with integrated control measures is recommended to reduce production losses
associated with avian coccidiosis. |
Citations
Copy and paste a formatted citation or use one of the links to import into a bibliography manager and reference.
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IEEE
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Thakare Jatinbhai Ramchandrabhai , Maulik rajvi, Dhwani R. Oza, Nilima N. Brahmbhat , Vandip D. Chauhan, "MOLECULAR DETECTION AND SPECIES IDENTIFICATION OF EIMERIA SPP. IN BROILER FARMS NEAR BY ANAND, GUJARAT, INDIA", International Journal Of All Research Writings,
vol. 8, no. 1, pp. 1-5, 2026.
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MLA
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Thakare Jatinbhai Ramchandrabhai , Maulik rajvi, Dhwani R. Oza, Nilima N. Brahmbhat , Vandip D. Chauhan "MOLECULAR DETECTION AND SPECIES IDENTIFICATION OF EIMERIA SPP. IN BROILER FARMS NEAR BY ANAND, GUJARAT, INDIA." International Journal Of All Research Writings,
vol 8, no. 1, 2026, pp. 1-5.
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APA
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Thakare Jatinbhai Ramchandrabhai , Maulik rajvi, Dhwani R. Oza, Nilima N. Brahmbhat , Vandip D. Chauhan (2026). MOLECULAR DETECTION AND SPECIES IDENTIFICATION OF EIMERIA SPP. IN BROILER FARMS NEAR BY ANAND, GUJARAT, INDIA. International Journal Of All Research Writings,
8(1), 1-5.
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MOLECULAR DETECTION AND SPECIES IDENTIFICATION OF EIMERIA SPP. IN BROILER FARMS NEAR BY ANAND, GUJARAT, INDIA
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MOLECULAR DETECTION AND SPECIES IDENTIFICATION OF EIMERIA SPP. IN BROILER FARMS NEAR BY ANAND, GUJARAT, INDIA
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